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In the course of search antagonistic fungi from soil in green house, four kind of fungi (AF1, AF2, AF3, AF4) were isolated, which have activities aganist Phytophthora capsici, Botrytis cinera, Rhizoctomia solani, Pythium ultimum and Fusarium oxysporum. The AF2 was identified according to the morphological description of Aspergillus terreus. This antagonistic fungus inhibiting various plant pathogens was effective to reduce disease incidence of cucumber seedlings caused by mixed inoculum of Rhizoctonia solani, Pythium ultimum and Fusarium oxysporum. Antifungal compound I was isolated and purified by fresh chromatography from A. terreus. The ¹H and ^(13)C assignment of compound I was achieved from two-dimensional ¹H-¹H COSY, HMQC, HMBC with the add of homonuclear and heteronuclear double resonance experiment. The compound I was identified butyrolactone I (α-oxo-β-(p-hydroxyphenyl)-γ-(p-hydroxy-m-3,3-dimethyl-allylbenzyl)-γ-methoxy-carbonyl-γ-butyrolactone, C_(24)H_(24)O_7, M.W.=424).
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